Jafar rezaie, a researcher in Stem Cell biology, Assistant Professor, Solid Tumor Research Center ; STRC

Contact information

 Tell  : +984433195073

Cell phone: +989148548503

Email: Rezaie.j@urmia.ac.ir  J.rezaie88@gmail.com

.Address: Floor 3, Cellular and MolecularMedicine Institute., Shafa St., Ershad Blvd., Urmia, West Azarbaijan, Iran

 

         I am working in the field of Extracellular Vesicles (EVs) biology especially focused on exosome signaling pathway in cancer. EVs contribute to physiological and pathological processes in a specific manner. In the field of cancer biology, scientists have interested in studying EVs biology and as well in the application of EVs in diagnosis and treatment of cancers. My scientific interests have focused on the study of small vesicles called exosomes, secreted by angiogeneic and tumor cells, which play many roles in communications between tumors. Here, we are interested in lightening the key role of EVs in cancerous tissues In Vivo/In Vitro, and also following their bio-application.

 

Research Focus

 Tumor derived exosomes kinetic

  Biomarker in cancer diseases

 Cancer immunotherapy

 Exosome-based drug delivery

 

Research Team

Dr. Jabbari

Dr. Soraya

Dr. Rahbarghazi

Dr. Khaksar

Dr. Akbari

Mr.Mazhar

Mr. Aftab

 

Research Experience

 

Well-experienced in stem cell biology with focus on cell isolation, expansion and differentiation.

Experienced in the field of stem cells such as Mesenchymal, Endothelial, Myocytes, and CRI-D2 cells.

Experienced in Exosome signaling pathway and angiogenesis mechanism studies, method development, validation, and design of in vivo or in vitro models studies.

Well-experienced in proteomics methods and related softwares especially Western Blot analysis.

Well-experienced in genomics methods and related softwares especially real time PCR investigation.

Well-experienced in cell staining and tracking methods with focus on immunocytochemistry and immunofluorescence, flow cytometry and techniques.

Experienced in proposal writing and manuscript preparation.

 Familiar to a panel of softwares especially SPSS, GIMP, InkScape , Sketchup, Flow Jo,  Image  J, Oligo 7 , Perlprimer, Rotor Gene ,... 

 

 

 

 

 

What is exosomes? 

 

      Exosomes are a subtype of extracellular vesicles (EVs) that represent a new tool in cell to cell communication through shuttling bio-molecules between cells. Exosomes released from various stem cells and committed cell types in size of 30-120nm into extracellular matrix (ECM) following constitutive or induced state. According to studies, exosome can be found in bio-fluids such as plasma, breast milk, urine, semen, amniotic fluid, ascites fluid, cerebrospinal fluid, bile, saliva, bronchoalveolar lavage, and also urine. Interestingly, exosomes purification from various cells represent common endosomal pathway proteins for example tetraspanins (CD9, CD63, CD81), Alix, heat shock proteins (Hsc70 and Hsp90), and tumor susceptibility gene 101 (Tsg101). An intriguing aspect of exosomes, these EVs actively participate to transfer a lot of molecules the same as a pocket covering their lumen contents from several RNAases and proteases. Last proteomic and transcriptomic analyses have been employed to discover exosomes nucleic acid and protein cargoes as well as lipid contents. In addition, research into exosomes compositions reveal the presence of many lipid rafts, proteins, enzymes, and sugar chains on exosomes surrounding membrane that governing exosome function. In the past decade, scientific reports demonstrated the key role of these vesicles in normal physiological process and pathogenesis of tumor and non-tumor diseases. Intensive efforts are in processing to elucidate pivotal role of these EVs in pathogenesis of diseases, which could expectantly address dynamic of exosome signaling pathways and clinical application of them in diagnosis and therapeutic approaches. Exosomes from diverse cellular origins have been revealed to harbor common molecules present in the parent cell, and consequently could be used rather than stem cells to persuade effective therapy. Indeed, exosome-based therapy could probably improve several issues about the safety and practical limitations associated with the application of stem/progenitor cells, such as the risk of maldifferentiation. It seems that regardless of intensive research in this field, there are still a lot of unclear question about exosome signaling pathways and their clinical usage that need to be considered in the future investigations.

 

Future prospect:


Along with the important role of the EVs in diseases, preconditioning or engineering of EVs surface membrane or their cargoes may result in disease specificity and efficacy. It seems that EVs represents a remarkable alternative for regenerative medicine and may also for diagnosing  diseases as a novel biomarker. Further endeavors still need to understand how EVs affects target cells? And how to engineer EVs to improve their efficacy in regenerative medicine? Furthermore, EVs from which cells are an excellent applicable candidate for therapeutic approaches?